Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.
نویسندگان
چکیده
Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which expressed a primer-dependent glucosyltransferase synthesizing an insoluble glucan.
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ورودعنوان ژورنال:
- Infection and immunity
دوره 49 2 شماره
صفحات -
تاریخ انتشار 1985